The movie depicts DEIR-V cells (normal human fibroblasts Detroit 551 transduced with E1A, HRAS and VSV-G) that were treated with PBS pH 6.0 for 1 minute and then filmed for the subsequent ~25 minutes by phase contrast microscopy. Each frame of the movie is 23 seconds, the replay is 7 frames per second. The timer shows HRS:MIN:SEC.msec elapsed from the beginning of the movie. The green arrows indicate the cells that fused to produce binuclear heterokaryons. Some frames from this movie are shown in Fig. 1C.
Posted by BioTechniques on 2010-09-20 19:58:19
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